Evaluation of the New BD Max GC Real-Time PCR Assay, Analytically and Clinically as a Supplementary Test for the BD ProbeTec GC Qx Amplified DNA Assay, for Molecular Detection of Neisseria gonorrhoeae.
نویسندگان
چکیده
The new BD Max GC real-time PCR assay showed high clinical and analytical sensitivity and specificity. It can be an effective and accurate supplementary test for the BD ProbeTec GC Qx amplified DNA assay, which had suboptimal specificity, and might also be used for initial detection of Neisseria gonorrhoeae.
منابع مشابه
A two-site analytical evaluation of the BD Viper System with XTR Technology in Nonextracted Mode and Extracted Mode with seeded simulated specimens.
The BD ProbeTec CT Q(x) Amplified DNA Assay and the BD ProbeTec GC Q(x) Amplified DNA Assay (both BD Diagnostics, Sparks, MD) represent two new assays developed for use with the BD Viper System with XTR Technology (BD Diagnostics). These assays were built on the foundation of the former BD ProbeTec ET assays (BD Diagnostics) and its accompanying instrumentation. The study described below compar...
متن کاملClinical evaluation of the BD ProbeTec™ Neisseria gonorrhoeae Qx amplified DNA assay on the BD Viper™ system with XTR™ technology.
BACKGROUND The excellent sensitivity and specificity of commercially available nucleic acid amplification tests (NAATs) for the identification of Neisseria gonorrhoeae have been demonstrated. This study evaluated the performance of the BD ProbeTec™ N. gonorrhoeae Q (GCQ) Amplified DNA Assay on the BD Viper™ System with XTR™ Technology in a multicenter study. METHODS Specimens were collected a...
متن کاملComparison of the BD Viper System with XTR Technology to the Gen-Probe APTIMA COMBO 2 Assay using the TIGRIS DTS system for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens.
BACKGROUND Performances of the BD ProbeTec Chlamydia trachomatis (CT)/Neisseria Gonorrhoeae (GC) Q(x) Amplified DNA Assay reagents on a BD Viper System with XTR Technology and APTIMA COMBO 2 Assay reagents on a TIGRIS DTS platform, for detection of both CT and GC were compared. METHODS A total of 1018 first-void urine specimens were tested for the presence of CT and GC DNA using the 2 assays....
متن کاملGenital and extra-genital screening for gonorrhoea using the BD Probetec ET system with an in-house PCR method targeting the porA pseudogene as confirmatory test.
Diagnosing gonorrhoea from extra-genital as well as genital sites is important in managing this sexually transmitted disease. In this study we evaluated a screening procedure for Neisseria gonorrhoeae (GC) from all sample sites in a low-prevalence setting. A total of 69,252 specimens submitted for Chlamydia trachomatis testing were also examined for GC on the BD Viper™ platform using the BD Pro...
متن کاملEvaluation of the BD ProbeTec CTQ Amplified DNA Assay for Detection of the New Chlamydia trachomatis Variant in Sweden
OBJECTIVES The nvCT has a 377 bp plasmid deletion that has caused commonly used nucleic acid amplification tests to generate false negative results. In Swedish counties nvCT has been reported to account for up to 65% of all chlamydia cases in 2007. The aim was to evaluate the CTQ DNA amplification assay for detection of the new Chlamydia trachomatis variant (nvCT) in urine specimens from a labo...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 53 12 شماره
صفحات -
تاریخ انتشار 2015